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Jointed Goatgrass (Aegilops cylindrica Host) x Wheat (Triticum aestivum L.) Hybrids

Hybridization Dynamics in Oregon Wheat Fields

L. A. Morrisona, O. Riera-Lizarazub, L. Crémieuxb and C. A. Mallory-Smith*,b

a Herbarium, Dep. of Botany and Plant Pathology, Oregon State Univ., Corvallis, OR 97331-2902
b Dep. of Crop and Soil Science, Oregon St. Univ., Corvallis, OR 97331-3002



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Fig. 1. Eastern Oregon counties (shaded) comprising the primary area of jointed goatgrass infestation. Dots indicate areas for sites where hybrids were collected.

 


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Fig. 2. (a) Spikes of F1 hybrid and parents. From left to right: wheat, F1 (jointed goatgrass = female parent), F1 (wheat = female parent), and jointed goatgrass. (b) Roots of F1 plants with maternal jointed goatgrass spikelet attached at the crown (left) and maternal wheat seed attached at planting depth (right). (c) Spikes of hybrids grown from Oregon backcross seed showing range of variation from jointed goatgrass-like (left) to wheat-like (right). (d) Spike examples illustrating potential steps of introgression from F1 hybrid (first and second to right) via a jointed goatgrass-like backcross hybrid (center) toward jointed goatgrass (left).

 


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Fig. 3. Glu-A1, Glu-B1, and Glu-D1 HMW glutenin subunits found in soft-white wheat cultivars grown in Oregon with Payne number assignments and their respective subunit coding for the male parentage analysis.

 


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Fig. 4. (a) Coding for HMW glutenin parental patterns for jointed goatgrass (cyl) and wheat (aes) and their F1 hybrid (F1). Occasional presence of the By subunit band is indicated by "---". (b) SDS-PAGE gel of HMW glutenin patterns of jointed goatgrass (1), Madsen (2), Madsen x jointed goatgrass F1 synthetic hybrid (3,4), and molecular weight marker (M) in kilo Daltons.

 



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Fig. 5. (a) Coding for HMW glutenin patterns in backcross hybrid seed: F1-like pattern F1-1; wheat-like pattern aes-1; jointed goatgrass like patterns cyl-1 and cyl-2. When the By subunit band as indicated by "---" is absent, the F1-like and wheat-like patterns are designated F1-1 and aes-1, respectively; when it is present, the patterns are designated F1-2 and aes-2, respectively. (b) composite SDS-PAGE gel of HMW glutenin patterns of jointed goatgrass (1); Chinese Spring (5); F1-1 (4,8); aes-1 (6); aes-2 (7); cyl-1 (3); cyl-2 (2).

 


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Fig. 6. SDS-PAGE gel of HMW glutenin patterns for Oregon material from site 113b: jointed goatgrass (3); F1cyl hybrid seed (1,2); Chinese Spring (4).

 


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Fig. 7. HMW glutenin pattern distributions for backcross hybrid seed (a) by year for all seed; (b) by collection for all seed – SP (single-plant collection), Bulk (bulk plant collection); (c) by F1 female parentage—aes (wheat) or cyl (jointed goatgrass) seed-spikelet; (d) by site for the seed-spikelet subsample.

 





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