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Transgenic Dry Bean Tolerant to the Herbicide Glufosinate Ammonium

Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Final Av. W3 Norte, 70770-900 - Brasília-DF, Brazil

View larger version (40K): [in a new window]   Fig. 1. Diagram of the plasmid vector (pB5/35Sbar) used for transformation of P. vulgaris (inner circle). The two outer circles indicate the sequences that were introduced into transgenic events PHV119 and PHV122. The position of the primers used in PCR analysis and the probes for Southern blot analyses are also indicated with arrows.

View larger version (132K): [in a new window]   Fig. 2. Evaluation of PHV119 for glufosinate ammonium tolerance under field conditions. (a) nontransgenic sprayed with 400 g GA ha-1; (b) nonsprayed transgenic plants and (c) sprayed with 400 g GA ha-1 and (d) 800 g GA ha-1. (e) Detail of a transgenic plant sprayed with 400 g GA ha-1 and (f) 800 g GA ha-1. Photographs were taken 2 wk after herbicide treatment.

View larger version (56K): [in a new window]   Fig. 3. Southern blot of putative transformation events in the T0 (A and B) and T1 (C) generations. DNAs were digested with HindIII and probed with an internal fragment of the bar (A and C) and neo (B) genes. A and B: Lane 1 = transgenic event PHV119, Lane 2 = transgenic event PHV122, Lane 3 = nontransgenic plant, Lane 4 = plasmid vector pB5/35Sbar (50 pg). C: Lanes 1 through 3 = T1 plants from event PHV122, Lane 4 = nontransgenic plant, Lanes 5 through 7 = T1 plants from event PHV119, Lane 8 = plasmid vector pB5/35Sbar (50 pg). The size markers are indicated to the left of the figure.