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Chromosomal Polymorphism as Detected by C-Banding Patterns in Chilean Alfalfa Germplasm1

Gary R. Bauchan*, T. Austin Campbell and M. Azhar Hossain

USDA-ARS, Soybean Genomics and Improvement Laboratory, Beltsville Agricultural Research Center, Beltsville, MD 20705



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Fig. 1. C-banded karyotype of M. sativa subsp. sativa. Dots on the chromosomes do not represent a C-band but are artifacts of preparation. The bar represents one micrometer

 


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Fig. 2. Summary idiograms of the polymorphisms which were observed in the C-banded chromosomes of M. sativa subsp. sativa African (top) and Chilean (bottom). Solid bands represent bands which were always present in all four homologs. Shaded bands represent bands which were not always present in all four homologs. Open bands represent bands which occasionally were present (<4%).

 


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Fig. 3. Dendrogram from Ward's minimum variance cluster analysis of genetic distances among 36 alfalfa clones tracing to Chilean (CG) or African (AG) germplasm sources. Dendrograms based on chromosomal C-banding patterns of Homolog 1 of all chromosomes (A) and of Chromosome 8 only (B) are presented.

 


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Fig. 4. Multiple correspondence analysis of genetic distances, based on C-banding patterns of Chromosome 8 Homolog 1, among 36 alfalfa clones tracing to Chilean (CG) or African (AG) germplasm sources.

 





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