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Identification of Expressed Sequence Markers for a Major Gene-Rich Region of Wheat Chromosome Group 1 Using RNA Fingerprinting–Differential Display

Dep. of Agronomy and Horticulture, 362H Plant Science, P.O. Box 830915, Univ. of Nebraska-Lincoln, Lincoln, NE 68583-0915

View larger version (35K): [in a new window]   Fig. 1. C-banding pattern (left) (Gill et al., 1991) and ideogram (center) of chromosome 1BS of wheat in comparison with the consensus genetic map (right) (Van Deynze et al., 1995). Asterisk represents fraction length of the satellite. Black boxes represent C-bands on the chromosome. NOR, nucleolar organizer region.

View larger version (15K): [in a new window]   Fig. 2. Autoradiogram of an RNAF–DD gel. Lanes 1 and 2 represent RNAF patterns of deletion 1BS-4 and 1BS-19, respectively. One of the fragment bands present in 1BS-4 but missing in 1BS-19 is marked by an arrow. One of the fragment bands present in 1BS-19 but missing in 1BS-4 is marked by a bullet.

View larger version (98K): [in a new window]   Fig. 3. Gel-blot DNA hybridization of a positive clone (UNL12) on genomic DNA of group 1 nullisomic-tetrasomic lines, 1BS-4 and 1BS-19. Lane 1, Nulli 1A-Tetra 1B; Lane 2, Nulli 1B-Tetra 1D; Lane 3, Nulli 1D-Tetra 1A; Lane 4, 1BS-4; Lane 5, 1BS-19. Arrow represents B fragment band present in 1BS-4 and missing in 1BS-19.

View larger version (26K): [in a new window]   Fig. 4. Physical mapping of group 1 probes identified by RNAF–DD, on chromosome 1B. Asterisk represents fraction length of the satellite. represent markers for which B-genome band was not resolved. Black boxes represent C-bands on the chromosome.