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Identification and Confirmation of Aluminum Tolerance QTL in Diploid Medicago sativa subsp. coerulea

M. K. Sledge*,a, J. H. Boutonb, M. Dall'Agnoll, W. A. Parrottb and G. Kochertc

a The Samuel Roberts Noble Foundation, Ardmore, OK 73402
b Dep. of Crop and Soil Sciences, Univ. of Georgia, Athens, GA 30602-7272
c Dep. of Botany, Univ. of Georgia, Athens, GA 30602-7271



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  		Fig. 1. Frequency distribution of Al tolerance, as measured with a callus bioassay, in three F2 populations of M. sativa subsp. coerulea.

 


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  		Fig. 2. Segregation of Al tolerance associated RFLP marker UGAc502 in three F2 populations. A is HindIII-cut {lambda} DNA; B is the Al sensitive parent M. sativa subsp. coerulea 440501-2, showing the DS1 and DS2 alleles; C is the Al tolerant parent M. sativa subsp. coerulea 464724-25, showing the DT1 and DT2 alleles; D is F1 Al-3; E is F1 Al-4; F is F1 Al-5. Population One is an F2 population derived from selfing Al-3, Population Two is from Al-4, and Population Three is from Al-5.

 


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  		Fig. 3. Linkage groups 1 and 8 of the UGA Alfalfa RFLP map, showing the locations of markers UGAc471 and UGAc502, which are associated with Al tolerance.

 


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  		Fig. 4. Frequency distribution of aluminum tolerance, as measured with a callus bioassay, in a backcross population of M. sativa subsp. coerulea.

 




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