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AFLP Analysis of Enset Clonal Diversity in South and Southwestern Ethiopia for Conservation

^a Inst. of Biodiversity Conservation and Research, P.O. Box 30726, Addis Ababa, Ethiopia
^b Debub Univ., Awassa College of Agriculture, P.O. Box 5, Awassa, Ethiopia
^c Centre for Genetic Resources, the Netherlands, Plant Research Int., P.O. Box 16, 6700 AA Wageningen, the Netherlands

View larger version (27K): [in a new window]   Fig. 1. Geographic origin of the enset clones analyzed in the present study. Geographic areas are all located in the Southern Nation, Nationalities, and Peoples' Regional Government (SNNPR) Province of Ethiopia (enlarged within the figure).

View larger version (76K): [in a new window]   Fig. 2. Part of the amplified fragment length polymorphism autoradiograms of six enset clones analyzed in the present study for the primer combinations E + AA/M + CCA, E + AA/M + CCT, E + AG/M + CCA, and E + AG/M + CCT. These primer combinations were selected from a group of 12 that were tested on the six enset clones. Vernacular names of these clones are Chele bocho (1), Choro (2), Neche nobo (3), Gesh ariko (4), Neche epo (5), and Ketano (6). Approximate size range of the fragments shown in the figure is 300 to 100 bp from top to bottom.

View larger version (18K): [in a new window]   Fig. 3. Principal coordinate (PCO) plot of the 146 enset clones investigated in the present study. The plot is based on Jaccard's similarity coefficient, using the amplified fragment length polymorphism data for the primer combinations E + AA/M + CCA, E + AA/M + CCT, E + AG/M + CCA, and E + AG/M + CCT. The percentage of the total variation explained by each axis is given in parentheses. Clones are denoted by symbols, representing their different geographic origin (Chena, Decha, Hadiya, Sidama, or Wolaita) in Ethiopia.