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Development and Characterization of Microsatellite and RFLP-Derived PCR Markers in Oat

^a Dep. of Crop Sciences, 1102 South Goodwin Ave., Univ. of Illinois, Urbana, IL 61801
^b USDA-ARS, Dep. of Crop Sciences, 1102 South Goodwin Ave., Univ. of Illinois, Urbana, IL 61801

View larger version (49K): [in a new window]   Fig. 1. Examples of polymorphisms observed between oat lines with microsatellite and RFLP-derived PCR markers. (A) Microsatellite markers 83, 87, AM102, and AM112. (B) STS markers derived from CDO270 (one of two loci ± polymorphic) and BCD1882 (two of three loci ± polymorphic). (C) Microsatellite marker derived from CDO270 and CAPS markers derived from BCD1407 and BCD1950 cleaved with MseI and MboI, respectively. The names of the primers are listed above each image. The oat lines analyzed are indicated above each lane K = Kanota, O = Ogle, C = Clintland 64, and I = IL86-5698.

View larger version (33K): [in a new window]   Fig. 2. Linkage map of PCR markers in the Kanota x Ogle recombinant inbred line population. Map distances are given in centimorgan (Kosambi function). Markers added in this study are shown in bold. Markers in parentheses have been assigned to intervals only. The positions of quantitative trait loci for tolerance to barley yellow dwarf virus are indicated with hatched boxes. In the lower right portion of the figure, the relative map positions of PCR markers derived from CDO270 in the Kanota x Ogle (KxO) and Clintland 64 x IL86-5698 (CxI) populations are shown.