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Highly Efficient Doubled-Haploid Production in Wheat (Triticum aestivum L.) via Induced Microspore Embryogenesis

Weiguo Liu*, Ming Y. Zheng, Enrique A. Polle and Calvin F. Konzak

Northwest Plant Breeding Company, 2001 Country Club Road, Pullman, WA 99163



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  		Fig. 1. Process of isolated microspore liquid culture for doubled-haploid production, genotype Chris. (a) Mid- to late-uninucleate microspores from the freshly harvested spikes. (b) Embryogenic microspores with fibrillar cytoplasm induced by 2-HNA treatment at high temperature for 65 h. (c) Microspore derived developing embryoids cultured in liquid media for 18 d. (d). Continuous production of mature embryoids upon liquid culture from 21 d on. (e) Germination of embryoids on solid media in Petri dish at 10 d. (f) Thousands of doubled-haploid plants derived from microspores of a single spike.

 




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