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A PCR Assay for Rapid Discrimination of Sterile Cytoplasm Types in Maize

Institute of Plant Sciences, Swiss Federal Institute of Technology, CH-8092 Zurich, Switzerland

View larger version (20K): [in a new window]   Fig. 1. Recombinant mtDNA regions of the sterile cytoplasm types T, C, and S. Black arrows indicate the binding sites of the specific primer pairs; the sizes of the expected amplification products are given between the arrows.

View larger version (61K): [in a new window]   Fig. 2. Identification of the three sterile cytoplasm types in the inbred line B37 determined by PCR from crude seed DNA as template.