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Simulation Experiments on Efficiencies of Gene Introgression by Backcrossing

^a CIMMYT, Int. Maize and Wheat Improvement Center, Lisboa 27, Apdo. Postal 6-641, 06600 Mexico D.F., Mexico
^b Monsanto Life Sciences Research Center, 700 Chesterfield Parkway North, St. Louis, MO 63198

View larger version (33K): [in a new window]   Fig. 1. Expected selection intensity as a function of population size with one, two, four, and eight individuals selected per generation, assuming an underlying normal distribution of donor genome contribution among screened genotypes. (A) Donor genome contribution at nontarget loci after BC1 for different values of selectable population size (Nsl) and for the transfer of one or five target genes. (B) For this calculation, 10 chromosomes of 200 centimorgans (cM) (total genome size of 2000 cM) were considered with a DNA marker each 10 cM. The range of the sample size between vertical lines represents the most cost effective sample size.

View larger version (17K): [in a new window]   Fig. 2. Donor genome contribution for the introgression at one target locus when (A) a complete marker-assisted selection (MAS) step [selectable population size (Nsl) = 100] is conducted during each of the three backcross (BC) generations, and (B) partial MAS is conducted during the first two BC generations (Nsl = 10) and a complete MAS is conducted at the third BC generation (Nsl = 100).