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Genetic and Sequence Analysis of Markers Tightly Linked to the Soybean mosaic virus Resistance Gene, Rsv3

^a Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg, VA 24061-0404
^b Monsanto Company, 3302 SE Convenience Blvd., Ankeny, IA 50021
^c USDA-ARS Small Fruits Research Unit, 306 S. High St., Poplarville, MS 39470

View larger version (18K): [in a new window]   Fig. 1. Genetic map of the soybean molecular linkage group B2 surrounding the soybean mosaic virus resistance gene, Rsv3. Markers were mapped in two segregating populations: (A) the cross L29 (Rsv3) x Lee68 (rsv3); (B) the cross Tousan 140 (Rsv3) x Lee68 (rsv3).

View larger version (68K): [in a new window]   Fig. 2. Partial sequence of the clones used for generating PCR-based markers. A. A519 sequence from L29 (top) and Lee68 (bottom). Forward and reverse primers for A519F/R are underlined. Nucleotides at substitution sites and indel site are shown in bold face. B. M3a sequence from a genomic clone of L81-4420. Forward and reverse primers for M3aSatt are underlined. Nucleotides in microsatellite regions are shown in bold face.

View larger version (27K): [in a new window]   Fig. 3. Predicted amino acid sequence of part of M1a determined on the basis of the nucleotide sequence of a genomic clone derived from the soybean line L81-4420. The deduced protein has been divided into two domains: A. putative N-terminal sequence; B. Leucine rich repeat (LRR). The amino acids corresponding to the consensus sequence of the extracellular LRR are shown in bold face. Aliphatic amino acids in the conserved sites are also shown in bold face.