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Ploidy Determination in Agrostis Using Flow Cytometry and Morphological Traits

Stacy A. Bonos*, Karen A. Plumley and William A. Meyer

Dep. of Plant Science, 59 Dudley Road, Foran Hall, New Brunswick, NJ 08901-8520



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Fig. 1. Microscopic views of grass root tips in metaphase indicating chromosome numbers of (A) velvet bentgrass (2n = 2x = 14), and (B) creeping bentgrass (2n = 4x = 28), magnification at x1000.

 


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Fig. 2. Histogram of propidium iodide fluorescence intensity of leaf tissue from Agrostis species indicating relative differences in signal intensity of Agrostis ploidy levels compared with the internal standard.

 





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