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Right arrow Germplasm Enhancement

Attempts to Transfer Russian Wheat Aphid Resistance from a Rye Chromosome in Russian Triticales to Wheat

Adam J. Lukaszewski*,a, David R. Porterb, Cheryl A. Bakerb, Krystyna Rybkac and Boguslaw Lapinskic

a Dep. of Botany and Plant Sciences, University of California, Riverside, CA 92521
b USDA-ARS, 1301 N. Western Rd., Stillwater, OK 74075-2714
c Institute of Plant Breeding and Acclimatization, 05-870 Blonie, Poland



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Fig. 1. Rye chromosome 4Rmon from PI 386148 and its translocations with wheat produced in this study. (a) C-banded chromosomes, from left to right: 4Rmon, 7D (of Pavon), 7DS.4Rmon and 7DS.4Rmon–2. (b) Fluorescent in situ DNA hybridization to chromosomes, from left to right: 4Rmon, 7DS.4RLmon, 7DS.4RLmon–1, and 7DS.4RLmon–2.

 


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Fig. 2. Meiotic metaphase I pairing in a heterozygote of the translocation 7DS.4RLmon–1. Quadrivalents are arrowed. a, phase contrast; b and c, fluorescent in situ hybridization.

 


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Fig. 3. Fluorescent in situ DNA hybridization to mitotic chromosomes of 97RWA936-5-1; 44 chromosomes including one pair of 4Rmon and one pair of centric translocations, presumably 5DS.5RLmon (enlarged from a different cell in the inset).

 





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