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DNA Content and Ploidy Determination of Bromegrass Germplasm Accessions by Flow Cytometry

Metin Tunaa, Kenneth P. Vogel*,b, K. Arumuganathanc and Kulvinder S. Gilld

a Dep. of Agronomy, Tekirdag Agriculture Faculty, Univ. of Trakya, Tekirdag, Turkey
b USDA-ARS, Wheat, Sorghum, and Forage Res. Unit, 344 Keim Hall, Univ. of Nebraska, P.O. Box 830937, Lincoln, NE 68507-0937
c Center for Biotechnology, Univ. of Nebraska, Lincoln, NE 68588
d Dep. of Agronomy, Univ. of Nebraska, Lincoln, NE 68583-0915



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Fig. 1. Bromus biebersteinii chromosomes from root-tip preparations. (A) Octaploid (2n = 56; PI 325226); (B) Decaploid (2n = 70; PI 341222)

 


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Fig. 2. Bromus inermis chromosomes from root-tip preparations. (A) Tetraploid (2n = 28; PI 315385); (B) Octaploid (2n = 56; Lincoln bromegrass).

 


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Fig. 3. Octaploid B. inermis ssp. pumpellianus chromosomes (2n = 56; PI 562648) from root-tip preparations.

 


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Fig. 4. Bromus riparius chromosomes from root-tip preparations. (A) Diploids (2n = 14; PI 440215); (B) Octaploid (2n = 56; PI 315380); (C) Decaploid (2n = 70; PI 536013).

 





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