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A Microsatellite Marker for Tagging Dn2, a Wheat Gene Conferring Resistance to the Russian Wheat Aphid

^a Dep. of Human Medical Genetics, Univ. of Colorado Health Sciences Center, Denver, CO 80262
^b TUBITAK Marmara Res. Center, The Res. Inst. for Genetic Engineering and Biotechnology, P.O. Box 21, 41470, Gebze-Kocaeli, Turkey
^c Dep. of Soil and Crop Sciences, Colorado State Univ., Fort Collins, CO 80523

View larger version (17K): [in a new window]   Fig. 1. Linkage map of wheat chromosome 7D containing the Russian wheat aphid resistance gene, Dn2. Genetic distances are given in cM. The marker Xgwm111 was mapped at a log of the odds (LOD) score of <2, while the rest were mapped at a LOD score of 3.

View larger version (88K): [in a new window]   Fig. 2. Silver-stained polyacrylamide gel showing polymerase chain reaction (PCR) amplification products of microsatellite Xgmw437 from DNA of B, six resistant F2 progeny; C, six susceptible F2 progeny; D, PI 262660 parental check; E, PI 262660 plant containing 104 base pairs (bp) as the highest band (Type I); F, PI 262660 plant containing 102 bp as the highest band (Type II); G, PI 262660 plant containing 100 bp as the highest band (Type III); H, a resistant F3 progeny; I, a susceptible F3 progeny; and J, a resistant progeny heterozygous for Xgwm437 which was not among the 6 resistant F2 bulked in lane B. Lane A contains a 10-bp DNA ladder (Gibco BRL, Rockville, MD).

View larger version (60K): [in a new window]   Fig. 3. Silver-stained polyacrylamide gel showing PCR amplification products of microsatellite Xgmw437 from DNA of various wheat cultivars. The amplification products of Xgwm437 from PI 262660 are distinct from those in susceptible cultivars and resistant cultivars containing genes other than Dn2. The first lane contains a 10-bp DNA ladder (Gibco BRL, Rockville, MD).