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Meiotic Stability, Chloroplast DNA Polymorphisms, and Morphological Traits of Upland x Lowland Switchgrass Reciprocal Hybrids

^a Univ. Autónoma Agraria Antonio Narro, Buenavista, Saltillo, Coah. México
^b USDA-ARS, 344 Keim Hall, Univ. of Nebraska, P.O. Box 830937, Lincoln, NE
^c Agronomy Dep., Univ. of Nebraska, Lincoln, NE 68583-0915

View larger version (119K): [in a new window]   Fig. 1. Pubescence in the upper base of the leaf blade of parents and progenies of a female lowland and a male upland switchgrass hybridization. The parents Kanlow (female, left) and Summer (male, right) are shown in the top row and the progeny in the subsequent rows. The Summer (female) and Kanlow (male) cross produced similar results (not shown).

View larger version (94K): [in a new window]   Fig. 2. Shape of seed of lowland by upland direct and reciprocal crosses. Top row: Kanlow (female, left), Summer (male, right); second row: progeny from the direct cross; third row: Summer (female, left), Kanlow (male, right); and fourth row: progeny from the reciprocal cross.

View larger version (131K): [in a new window]   Fig. 3. Microsporocyte of Kanlow by Summer hybrid showing 18 bivalent pairs at diakinesis stage of meiosis.

View larger version (133K): [in a new window]   Fig. 4. Microsporocyte of Kanlow by Summer hybrid showing 17 bivalents and two univalents at diakinesis stage of meiosis.

View larger version (48K): [in a new window]   Fig. 5. Chloroplast DNA polymorphism among parents and hybrids of switchgrass. DNA was digested with BamHI restriction endonuclease and hybridized with the spinach cpDNA probe pRR12. Lanes A and B are the Summer female and Kanlow male parents, respectively, of the progeny in Lanes C to F. Lanes H and I are the Kanlow female and Summer male parents, respectively, of the progeny in Lanes J to N. Lane O is the Kanlow male parent of the progeny in Lanes P to R. Lane S could not be verified due to death of the source plant. In the last lane on the right the molecular weight of polymorphic bands of HindIII-cut DNA are marked in kilobases (kb).