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Loss of Kernel Set Due to Water Deficit and Shade in Maize

Carbohydrate Supplies, Abscisic Acid, and Cytokinins

Tim L. Setter*, Brian A. Flannigan and Jeff Melkonian

Dep. of Crop and Soil Sciences, Cornell Univ., 521 Bradfield Hall, Ithaca, NY 14853



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Fig. 1. Mature kernel size and number in maize plants subjected to 5-d water deficit treatments during pre-pollination and post-pollination stages in Exp. I. (a): Kernel dry matter yield per plant, (b): kernel number per ear, (c): average kernel size for all kernels on an ear (left) and in apical regions of ears at the 35th and 40th nodal rings, counting from the base of each ear. Averages ± SEM of seven replicates are shown.

 


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Fig. 2. Carbohydrate concentrations per gram FW in tissues sampled from the apical ear region of plants subjected to control and 5-d water deficit treatments during pre-pollination (upper panels) and post-pollination (lower panels) stages in Exp. I. Means ± SEM of seven replicates are shown.

 


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Fig. 3. Abscisic acid concentrations in per gram FW in tissues sampled from the apical ear region of plants subjected to control and 5-d water deficit treatments during pre-pollination (left) and post-pollination (right) stages in Exp. I. Means ± SEM of seven replicates are shown.

 


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Fig. 4 Leaf gas exchange of plants subjected to control (•), shade ({Delta}) and water deficit ({square}) treatments during pre-pollination (upper panels) or post-pollination (lower panels) developmental stages in Exp. II. Net photosynthetic CO2 exchange rate (Pn; left panels) and stomatal CO2 conductance (gs; right panels) of 3 to 5 replicates (mean ± SEM) are shown. Data are plotted with respect to the days from treatment termination (DFTT). On the days of treatment termination (0 DFTT), plants were re-watered or removed from shade at 0800 and gas exchange data was acquired at 1100 to 1400 h.

 


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Fig. 5. Carbohydrate concentrations in florets (upper panels) and vascular cobs (lower panels) of plants subjected to control, shade, and water deficit treatments during the pre-pollination stage in Exp. II. Tissues were sampled from apical and basal regions of ears at the conclusion of treatments. Means ± SEM of 10 replicates are shown.

 


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Fig. 6. Carbohydrate concentrations in endosperm/nucellus composite tissues (upper panel) and pedicels (lower panels) of plants subjected to control, shade, and water deficit treatments during the post-pollination stage in Exp. II. Tissues were sampled from apical and basal regions of ears at the conclusion of treatments, 7 d after pollination. Means ± SEM of 10 replicates are shown.

 


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Fig. 7. Total nonstructural carbohydrate accumulation during 5-d shade and water deficit treatments in florets sampled at the pre-pollination stage (a) and composite endosperm/nucellus tissues sampled at the post-pollination stage (b). Nonstructural carbohydrates was calculated as the change during the 5-d stress period of the sum of glucose, fructose, sucrose, and starch in tissues.

 


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Fig. 8. Abscisic acid concentrations in florets (a), vascular cobs (b), endosperm/nucellus composite tissues (c) and pedicels (d) of plants subjected to control, shade, and water deficit treatments during the pre-pollination stage (upper panels) and post-pollination stage (lower panels) in Exp. II. Tissues were sampled from apical and basal regions of ears at the conclusion of treatments. Means ± SEM of 10 replicates are shown.

 


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Fig. 9. Total zeatin-like cytokinin concentrations in florets (a), vascular cobs (b), endosperm/nucellus (c) and pedicels (d) of plants subjected to control, shade, and water deficit treatments during the pre-pollination stage (upper panels) or post-pollination stage (lower panels) in Exp. II. Means ± SEM of 10 replicates are shown.

 





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