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Marker-Assisted Backcrossing for Introgression of a Recessive Gene

Institute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70953 Stuttgart, Germany

View larger version (6K): [in a new window]   Fig. 1. Chromosome of length L with the target locus at position x, two markers for foreground selection at positions ml and mr, and two markers for background selection at positions yl and yr. The map distances of the target locus to the foreground selection markers are denoted with 1 and 2 and those to the background selection markers with d1 and d2.

View larger version (28K): [in a new window]   Fig. 2. Probability qg that at least one BC progeny with genotype t+ T+ is generated by backcrossing individuals of Genotype g G0. In the left diagram (A), the family size fg derived from one backcrossed individual (ig = 1) is increased. In the right diagram (B), the number of backcrossed individuals ig is increased for family size fg = 1. Marker distances are d1 = d2 = 0.10 M and 1 = 2 = 0.02 M.

View larger version (23K): [in a new window]   Fig. 3. Estimates of the expected total number of individuals n1 + E(n2) required in a two generation backcross program (marker distances: 1 = 2 = 0.02 M, d1 = d2 = 0.10 M) in order to generate with probability q2 = 0.900, 0.990, and 0.999 at least one BC2 individual with genotype t+ T+. The values depend on the probability q1 of obtaining at least one BC1 individual with genotype g+ G+.