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Right arrow Crop Genetics

Efforts to Initiate Construction of a Disease Resistance Package on a Designer Chromosome in Tobacco

Ramsey S. Lewis and Earl A. Wernsman*

Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620



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  		Fig. 1. Percentage transmission of methotrexate resistance in BC1F1 families. Transmission of methotrexate resistance was determined by seeding 121 to 968 seeds per family on medium containing 1 mg L-1 methotrexate.

 


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  		Fig. 2. PCR assay to detect the presence or absence of the N transgene in MtxR GH96-3700 BC1F1 individuals from the cosegregation analysis. Reactions for TMVR plants were expected to amplify a 540-base pair (bp) N-gene fragment. Lanes 1 and 28, 100 bp ladder; Lanes 2 and 3, untransformed TMVS check genotypes Petite Havana and NC152-dhfr; Lanes 4 through 27, 24 different BC1F1 individuals. Comparative results from the TMV test are provided at the top of the panel.

 


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  		Fig. 3. Southern blot for MtxR GH96-3700 BC1F1 individuals from the cosegregation analysis. DNA was restricted with XbaI and hybridized with a N specific probe. Positions of DNA size markers are given to the left of the panel. Lanes 1 and 2, untransformed TMVS check genotypes Petite Havana and NC152-dhfr; Lanes 3 through 12, 10 different BC1F1 individuals. Comparative results from the TMV test and PCR assay are provided at the top of the panel.

 




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