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Fig. 1. Autoradiographs of 32P-labeled SSR-containing PCR products amplified from genomic DNA and separated on DNA sequencing gels. Panel A: SSR marker analysis at the Sat_090 locus of 32 F2 plants from a Triplo 13 F1 hybrid. The male parent of the F1 lacked lipoxygenase (lx1lx1). The F2 plants are classified as "A" (homozygous for what was assumed to be the female parent allele), "H" (heterozygous), or "B" (homozygous for what was assumed to be the male parent allele). F2 plant numbers 1, 28, and 33 were grown from F2 seeds that lacked lipoxygenase (lx1lx1). Panel B: SSR marker analysis at the Satt300 locus of the parents and 28 F2 plants from a Triplo 5 F1 hybrid. The Triplo 5 female parent was similar to Clark 63 (Clk) and the male parent (P1) was an experimental line in which seed urease was absent (urease null). The F2 plants are classified as "A" (homozygous for female parent allele), "H" (heterozygous), or "B" (homozygous for male parent allele). F2 plant number 30 produced seed that lacked urease (eu1eu1).
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