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DNA Endoreduplication in Maize Endosperm Cells is Reduced by High Temperature During the Mitotic Phase

Dep. of Agronomy and Plant Genetics, Univ. of Minnesota, 1991 Upper Buford Circle, St. Paul, MN 55108

View larger version (44K): [in a new window]   Fig. 1. The effect of sequential application of high temperature treatments (HTTs) (4 d at 35°C) on maize endosperm fresh weight (FW). Endosperms were weighed at the time of preparation for flow cytometry (FCM). Different letters indicate a significant difference of means, n = 18, LSD = 0.05.

View larger version (56K): [in a new window]   Fig. 2. The effect of sequential application of high temperature treatments (HTTs) (4 d at 35°C) on maize endosperm nuclei number (2A) and mitotic index (2B). Nuclei number was determined by flow cytometry (FCM). Different letters indicate a significant difference of means, n = 6, LSD = 0.05. Mitotic index was determined as the number of nuclei in prophase, metaphase, anaphase, and telophase, divided by the total number of nuclei viewed. Different letters indicate a significant difference of means, n = 6 endosperms, and 300 nuclei per endosperm were counted, LSD = 0.05.

View larger version (64K): [in a new window]   Fig. 3. The effect of sequential application of high temperature treatments (HTTs) (4 d at 35°C) on the percent nuclei with a mitotic DNA content (3A), and on the percent nuclei with an endoreduplicating DNA content (3B). The mitotic DNA class was comprised of the 3 and 6 C DNA content classes. The endoreduplication DNA classes were compiled by adding the percent nuclei in the 12, 24, 48, and 96 C classes. Different letters indicate a significant difference of means, n = 6, LSD = 0.05.

View larger version (42K): [in a new window]   Fig. 4. The effect of sequential application of high temperature treatments (HTTs) (4 d at 35°C) on average DNA content in maize endosperm. Different letters indicate a significant difference of means, n = 6, LSD = 0.05.