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Multipurpose Cryogenic Surface Apparatus

A Liquid Nitrogen-Chilled Sample Tray

U.S. Water Conservation Laboratory, USDA-ARS, 4331 East Broadway, Phoenix, AZ 85040

View larger version (32K): [in a new window]   Fig. 1. Diagram of multipurpose cryogenic surface apparatus.

View larger version (104K): [in a new window]   Fig. 2. (a) View of multipurpose cryogenic surface working surface and thermometer; temperature readout is -188.3°C. (b) Measurement of leaf area with digital calipers.

View larger version (17K): [in a new window]   Fig. 3. (a) Initial and (b) full activity of sorghum leaf ribulose-1,5-bisphosphate carboxylase and (c) phosphoenol pyruvate carboxylase activity after designated period of thawing or time on the multipurpose cryogenic surface apparatus. Error bars present for all data points, but may be hidden by symbols.

View larger version (63K): [in a new window]   Fig. 4. Sodium dodecyl sulfacte polyacrylamide gel electrophoresis of sorghum leaf proteins. Lane 0, molecular markers; Lane 1, upon removal from liquid N2; Lane 2, after 1 min of thawing; Lane 3, after 2 min thawing; Lane 4, after 5 min thawing; Lane 5, after 10 min thawing; and Lane 6, after 10 min exposure on multipurpose cryogenic surface apparatus. Abbreviations: R LSU, ribulose-1,5-bisphosphate carboxylase (Rubisco) large subunit; LHCII, light-harvesting complex II; R SSU, Rubisco small subunit.