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Quantitative Evaluation of Genetic Diversity in Wheat Germplasm Using Molecular Markers

M.M. Manifestoa, A.R. Schlattera, H.E. Hoppb, E.Y. Suáreza and J. Dubcovsky*c

a Laboratorio de Marcadores Moleculares, Instituto de Recursos Biológicos CIRN-INTA, 1712 Castelar, Buenos Aires, Argentina
b Instituto de Biotecnología CICVyA-CNIA, INTA 1712 Castelar, Buenos Aires, Argentina
c Dep. of Agronomy and Range Science, Univ. of California, Davis, CA 95616



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  		Fig. 1. Bread wheat genotypes assayed with SSR locus Xpsp2999 = locus Glu-A3 (1AS) and Xpsp3000 = Gli-B1 (1BS). Xpsp2999 amplified a single major band and Xpsp3000 two (see arrows). Each main band has "stutter" fainter bands separated by 3 bp produced by errors of the polymerase during the amplification of these trinucleotide SSRs. The size of the main bands in base pairs is detailed at the bottom of the figure.

 


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  		Fig. 2. Dendrogram of 67 bread wheat cultivars from Argentina based on 10 SSR and 71 AFLP fragments. Only cultivars with known pedigree were included in this dendrogram. Values in the X-axis correspond to Dice coefficients of similarity. Numbers in italics between brackets indicate breeding programs: (1) Inta, (2) Buck, (3) ACA, (4) Klein, (5) Thomas, (6) Northrup King, and (7) Dekalb.* Cultivar ‘Ciano’ was released by CIMMYT and ‘Olaeta Artillero’ by I. Vigliano (these two cultivars were not included in the analysis of Table 3).

 




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