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Incidence and Diversity of Neotyphodium Fungal Endophytes in Tall Fescue from Morocco, Tunisia, and Sardinia

S.L. Clementa, L.R. Elbersona, N.N. Youssefb, C.M. Davittc and R.P. Dossd

a USDA-ARS, Plant Germplasm Introduction and Testing Research Unit, Washington State Univ., Pullman, WA 99164-6402
b Department of Plant, Soil, and Entomological Sci., Univ. of Idaho, Moscow, ID 83843
c Electron Microscopy Center, Washington State Univ., Pullman, WA 99164-4210
d USDA-ARS, Horticultural Crops Research Unit, Corvallis, OR 97330



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  		Fig. 1. Scanning electron microscope photomicrographs of conidia of Neotyphodium isolates from tall fescue accessions W6 15860 (Morocco) (A), W6 16139 (Sardinia) (B), W6 16206 (Sardinia) (C), and W6 16079 (Tunisia) (D). Scale bars = 3 µm

 


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  		Fig. 2. Ethidium bromide-stained agarose gel showing 444 base pair (bp) amplification product obtained after polymerase chain reaction with tubulin-2 primers and DNA from Neotyphodium-infected and Neotyphodium-free plant tissue from Sardinia (panel A) and Tunisia (panel B) tall fescue accessions. Tall fescue accession and plant number designations are indicated above lanes (for example, accession 16153 and plant number 3 as 16153-3). Molecular weight standards are in the leftmost lane. C-16 and C-8 (panel A) and C-16 and C-9 (panel B) represent plants of a commercial tall fescue line (cv. Tribute). A Neotyphodium-specific band is evident for each of the endophyte-infected plants, namely C-16, 16153-3, 16206-5, 16139-5, 16150-5, and 16188-4 in panel A, and C-16, 15978-1, 16058-1, 16059-4, and 16079-7 in panel B. The absence of bands in lanes for C-8, 16206-1, 16150-1, and 16188-5 in panel A, and C-9 and 16079-5 in panel B reflects the endophyte-free status of these plants

 




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