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Incorporation of Sequenced cDNA and Genomic Markers into the Soybean Genetic Map

Benjamin F. Matthewsa, Thomas E. Devinea, Jane M. Weisemanna, Hunter S. Bearda, Kimberly S. Lewersa, Margaret H. MacDonalda, Yong-Bong Parkb, Rama Maitia, Jhy-Jhu Linc, Jonathan Kuoc, Monica J. Pedronia, Perry B. Cregana and James A. Saundersa

a Climate Stress Laboratory, USDA-ARS, Beltsville, MD 20705
b Cheju National Univ., Dep. of Horticulture, Cheju, Korea
c Life Technologies, Inc., Gaithersburg, MD 20877



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Fig. 1. Soybean genetic linkage map generated from a 149 individual F2 population derived from cv. Noir 1 (PI 290136) and cv. BARC-2 (Rj4) (PI 547895). Because this work emphasized placement of sequenced cDNA and genomic markers of known identity, only linkage groups containing these markers are represented. Six of the cDNA clones represent genes of known function, and the genes they represent are indicated in parentheses near the cDNA clone marker locus. Seven cDNA clones have detected multiple polymorphisms in this or other populations, and loci derived from those cDNA clones are designated to indicate the cDNA clone that identified them followed by the locus specification as published previously (for example pBLT24_1). SSR markers, in italics, were used to anchor linkage groups according to assignment in Cregan et al. (1999). AFLP markers are named according to Keim et al. (1997) (three EcoRI selective nucleotides, three MseI selective nucleotides, size in base pairs). Dashed lines in linkage groups represent statistically unlinked regions of known linkage groups reported by Cregan et al. (1999)

 





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