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Tracing the Phylogeny of the Hexaploid Oat Avena sativa with Satellite DNAs

Dep. of Plant Sciences & Crop Development Centre, Univ. of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8, Canada

View larger version (36K): [in a new window]   Fig. 1 Alignment of the three 195-bp repeats in the satellite sequence ASS49. Each repeat contains four monomers with an average of 49 bp. An 8-bp inverse repeat is overlined in the monomer

View larger version (98K): [in a new window]   Fig. 2 Southern blot analysis of the DNAs of diploid, tetraploid, and hexaploid oats determined with the satellite sequence ASS49 as a probe. Species in lanes 1, A. longiglumis; 2, A. wiestii; 3, A. canariensis; 4, A. strigosa; 5, A. clauda; 6, A. abyssinica; 7, A. barbata; 8, A. maroccana; 9, A. murphyi; 10, A. sterilis; 11, A. fatua; 12, A. sativa. (a) Ethidium bromide stained 1% (w/v) agarose gel after separation of DNA samples cut with XbaI showing similar amounts of plant DNA in each lane. (b) Autoradiogram after a 12-h exposure

View larger version (113K): [in a new window]   Fig. 3 Directed amplification of minisatellite DNA region from 13 Avena species performed by means of a minisatellite core sequence of rice HVR as a primer. Species in lanes 1, A. longiglumis; 2, A. wiestii; 3, A. canariensis; 4, A. strigosa; 5, A. clauda; 6, A. abyssinica; 7, A. barbata; 8, A. maroccana; 9, A. murphyi; 10, A. sterilis; 11, A. fatua; 12, A. byzantina; 13, A. sativa. Lane M is a molecular ladder

View larger version (25K): [in a new window]   Fig. 4 Prinicipal coordinate analysis of the Avena species based on the polymorphisms in minisatellites (a) and microsatellites (b)

View larger version (16K): [in a new window]   Fig. 5 Consensus phylogenetic tree of 12 Avena species based on the polymorphisms in minisatellites and microsatellites

View larger version (22K): [in a new window]   Fig. 6 A proposed evolutionary pathway of Avena species