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Marker-Based Selection of QTL Affecting Grain and Malt Quality in Two-Row Barley

^a Dep. of Plant Science, McGill Univ., 21111 Lakeshore, Ste-Anne-de-Bellevue, QC, Canada, H9X 3V9
^b Grain Research Laboratory, 1404-303 Main St., Winnipeg, MB, Canada R3C 3G8
^c Crop Development Centre, Univ. of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
^d Agriculture and Agri-Food Canada, P.O. Box 39088, St. John's, NF, Canada A1E 5Y7
^e Agriculture and Agri-Food Canada, Brandon, MB, Canada R7A 5Y3
^f Dep. of Plant Sciences, Univ. of Saskatchewan, Saskatoon, SK, Canada S7N 5A8
^g Monsanto, 67 Scurfield Blvd., Winnipeg, MB, Canada R3Y 1G4
^h Agriculture and Agri-Food Canada, Eastern Cereal and Oilseed Research Centre, Ottawa, ON, Canada K1A 0C6
ⁱ Dep. of Agricultural, Food and Nutritional Science, Univ. of Alberta, Edmonton, AB, Canada T6G 2P5
^j Dep. of Plant Agriculture, Univ. of Guelph, Guelph, ON Canada, N1G 2W1

View larger version (23K): [in a new window]   Fig. 1 Linkage maps of chromosomes 3, 6, and 7 (5H) of the Harrington/TR306 barley cross, oriented with the plus arms at the top of the figure, and showing the approximate centimorgan positions (Kosambi function) of marker loci (mostly RFLP). Markers indicated in bold type are those used to represent or flank the QTL regions in this study. The 5H chromosome designation is that proposed by Shepherd and Islam (1992)

View larger version (29K): [in a new window]   Fig. 2 Frequency distributions for mean extract ß-glucan and mean fine-coarse difference for four groups of doubled haploid lines. Marker genotypes defining the T-H, H-T, H-H, and T-T groups are given in Table 1

View larger version (46K): [in a new window]   Fig. 3 Schematic representation of selection for specific marker genotypes among 47 doubled haploid lines from the Harrington/TR306 cross. The doubled haploid lines are arranged in rank order on the basis of their phenotypes for mean extract ß-glucan and mean fine–coarse difference, from the most favorable at left to the least favorable at right. Shaded rectangles represent lines that would be selected on the basis of their marker genotypes in (A) QTL regions on chromosome 7 or (B) QTL regions on chromosomes 3, 6, and 7

View larger version (26K): [in a new window]   Fig. 4 Frequency distributions for three grain quality traits for four groups of doubled haploid lines. Marker genotypes defining the T-H, H-T, H-H, and T-T groups are given in Table 1

View larger version (25K): [in a new window]   Fig. 5 Frequency distributions for four malt quality traits for four groups of doubled haploid lines. Marker genotypes defining the T-H, H-T, H-H, and T-T groups are given in Table 1