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Mapping Yr28 and Other Genes for Resistance to Stripe Rust in Wheat

R.P. Singha, J.C. Nelsonb and M.E. Sorrellsc

a Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600 Mexico, D. F., Mexico
b 49 Lionel Rd., Darlington WA 6070, Australia
c Dep. of Plant Breeding and Biometry, 252 Emerson Hall, Cornell Univ., Ithaca, NY 14853, USA



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Fig. 1 Infection type (IT) distribution of Synthetic/Opata wheat recombinant inbred lines tested with two pathotypes of P. striiformis. The bars labeled "Cumul[ative]" R and S are inserted to illustrate the 3:1 and 1:1 segregation ratios for the pooled resistant vs. susceptible line classes

 


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Fig. 2 Chi-square contingency-test contours for seedling stripe rust IT scored as a binary (R, S) trait in Synthetic/Opata wheat RILs. Key to plot labels: 1 4E14_RS, response to race 14E14 at standard temperatures; Yr28, Yr18', resistance responses of these assigned genes to challenge with race MEX96-11. Markers are arrayed in map order with short arms of chromosomes at right. Chromosome lengths reflect numbers of markers, not genetic length

 


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Fig. 3 Contours showing LOD for simple regression of seedling IT in Synthetic/Opata wheat RILs on each RFLP marker in turn, after removal of resistant lines carrying the Synthetic allele at RFLP marker Xmwg634-4DS, and chi-square contingency test for the same trait coded as binary (R, S). Markers are arrayed in map order with short arms of chromosomes at right. Chromosome lengths reflect numbers of markers, not genetic length. In the upper (LOD) plot, deviation from center line at a marker indicates the parent contributing the allele associated with higher disease

 


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Fig. 4 Distribution of Synthetic x Opata wheat RILs for field stripe rust severity at two locations. Severity scores are based on the modified Cobb scale (Peterson et al., 1948)

 


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Fig. 5 LOD contours for simple regression of field stripe rust severity in Synthetic/Opata wheat RILs on each RFLP marker in turn. Markers are arrayed in map order with short arms of chromosomes at right. Chromosome lengths reflect numbers of markers, not genetic length. Deviation from center line at a marker indicates the parent contributing the allele associated with higher disease

 


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Fig. 6 Interval regression LOD contours for adult-plant stripe-rust resistance associated with four chromosome arms in Synthetic/Opata wheat RILs

 





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