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Inheritance of Multiple Transgenes in Wheat

^a Dep. of Agronomy, Univ. of Nebraska, Lincoln, NE 68583 USA
^b Dep. of Plant Pathology, Univ. of Nebraska, Lincoln, NE 68583 USA
^c Center for Biotechnology, Univ. of Nebraska, Lincoln, NE 68583 USA
^d Center for Biotechnology/Dep. of Agronomy, Univ. of Nebraska, Lincoln, NE 68583 USA

View larger version (16K): [in a new window]   Fig. 1 A partial map of plasmids (a) pRL, (b) p2-5A, and (c) pUbiNPTII-I used in the wheat transformation, including restriction sites used for Southern hybridization

View larger version (48K): [in a new window]   Fig. 2 (a) PCR amplification of a 1059 bp RNase L fragment in 14 T1 progeny segregating for RNase L: lanes: 1, 1 kb ladder; 2-15, T1 progeny; 16, skip; 17, pRL, positive control; 18, skip; 19, non-transformed plant, negative control. (b) PCR amplification of a 633 bp 2-5A synthetase fragment in 16 T1 progeny segregating for 2-5A synthetase: Lanes: 1, 1-kb ladder; 2-17, T1 progeny; 18, p2-5A, positive control; 19, non-transformed plant, negative control

View larger version (95K): [in a new window]   Fig. 3 Southern hybridization of RNase L in six segregating T2 testcross progeny. DNA was restricted with HindIII and hybridized with RNase L probe. The positions of DNA size markers are given to the left of the panel. Lanes: 1 through 6, T2 testcross progeny; 7 non-transformed plant, negative control; 8, pRL, positive control. Comparative results of the PCR amplification of RNase L are given at the top of the panel