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A Five-Minute Field Test for On-Farm Detection of Pre-Harvest Sprouting in Wheat

^a Australian Centre for International Agricultural Research, GPO Box 1571, Canberra ACT 2601 Australia
^b Quality Wheat CRC Limited, Private Bag 1345, North Ryde NSW 1670 and CSIRO Plant Industry, GPO Box 1600, Canberra ACT 2601 Australia

View larger version (28K): [in a new window]   Fig. 1 A. Principle of the test; B. Flow chart indicating test steps

View larger version (95K): [in a new window]   Fig. 2 Test card (open) showing inner surfaces and pads ready for addition of extract and buffer

View larger version (15K): [in a new window]   Fig. 3 Between-run precision of the immunochromatography tests; relationship between reflectance (P + P test), reflectance ratio (P + M test) and Falling Number. Data are mean ± SD for 14 hard wheat samples from Roma, Qld, analyzed on five separate days

View larger version (31K): [in a new window]   Fig. 4 Within-run precision of the immunochromatography tests; reflectance values for samples of FN 154, 275 and 382 for both test formats. Data are mean ± SD for samples analyzed five times on the same day

View larger version (45K): [in a new window]   Fig. 5 Effects of test time on A. reflectance and B. reflectance ratio for the P + P test. Data are means ± range for duplicate tests on six hard wheats from Roma, Qld

View larger version (11K): [in a new window]   Fig. 6 Cultivar influences on test performance: Relationship between Falling Number and reflectance (P + P test) for samples of five diverse wheat cultivars

View larger version (33K): [in a new window]   Fig. 7 Effect of ambient temperature on assay performance; data are means ± SD for reflectance values from the P + M assay for 14 hard wheat samples from Roma, Qld

View larger version (41K): [in a new window]   Fig. 8 Stability of antibody-coated test cards at 37°C. Data are reflectance values for A. P + P test, B. P + M test at day 1 (unstored) and after storage for 7, 21, and 28 d