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PCR-Based Markers Facilitating Marker Assisted Selection in Sunflower for Resistance to Downy Mildew

Lutz Brahm, Thomas Röcher and Wolfgang Friedt

Institute of Crop Science and Plant Breeding, Justus-Liebig-University, Ludwigstr. 23, D-35390 Giessen, Germany



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  		Fig. 1 Linkage map for the Pl2 locus in sunflower constructed by means of markers in coupling (a) and repulsion (b) phase

 


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  		Fig. 2 Segregation of SCAR marker SCAA111008 in the sunflower F2 population derived from the cross HA89(cms) x AS110Pl2. Lane 1, resistant parent AS110Pl2; Lane 2, susceptible parent HA89(cms); Lane 3, resistant bulk; Lane 4, susceptible bulk; Lane M, molecular weight marker (100-bp ladder, Gibco BRL). R = resistant, S = susceptible as tested in F3 (F2 progeny), - = no resistance data available because of sterility of F2 plant

 


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  		Fig. 3 Amplification of markers OPAA14750 (a) and OPAS12280 (b) in different resistant and susceptible sunflower germplasms. Lane 1, AS110; Lane 2, AS110Pl2; Lane 3, HA89; Lane 4, RHA325; Lane 5, RHA345; Lane 6, RHA348; Lane 7, CM587; Lane 8, CM592; Lane 9, CM596; Lane 10, CM610; Lane 11, HA291; Lane 12, HA323; Lane 13, HA342; Lane 14, HA350; Lane 15, HA850; Lane 16, HA-R2; Lane 17, CM591; Lane 18, CM594; Lane 19, CM603; Lane M, molecular weight marker (100-bp ladder, Gibco BRL). OPAA14750 and OPAS12280 are marked by arrows

 




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