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Host Variation in Traits Associated with Crown Nodule Senescence in Soybean

David Espinosa-Victoriaa, Carrol P. Vanceb and Peter H. Grahamc

a Programa Edafologia, Colegio de Postgraduados, Montecillo, Mexico
b USDA-ARS, Univ. of Minnesota, St Paul, MN 55108 USA
c Department of Soil, Water, and Climate, Univ. of Minnesota, St Paul, MN 55108 USA



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Fig. 1 Profiles of specific nodule activity for crown nodules of the soybean cultivars Alpha, Chippewa, Hardin, and Hodgson 78 from 10 to 66 d after emergence following inoculation with Bradyrhizobium japonicum strain UMR161. Nodule activity is measured as acetylene reduction to ethylene. Each point is the mean of three replicates, with vertical bars representing ± standard error

 


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Fig. 2 Expression of phenylalanine ammonia lyase (Fig. 2A) and chalcone synthase (Fig. 2B) in crown nodules of the soybean cultivars Alpha, Chippewa, Hardin, Hodgson 78, and Nod 1-3 from 10 to 59 d after emergence following inoculation with Bradyrhizobium japonicum strain UMR161

 


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Fig. 3 Protease activity in crown nodules of the soybean cultivars Alpha, Chippewa, Hardin, and Hodgson 78 from 10 to 59 d after emergence (DAE). Bars with the same letter at each DAE are not significantly different using Duncan's multiple range test ({alpha} = 0.05)

 


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Fig. 4 Leghemoglobin content of crown nodules of the soybean cultivars Alpha, Chippewa, Hardin, and Hodgson 78 from 10 to 59 d after emergence (DAE). Bars with the same letter at each DAE are not significantly different using Duncan's multiple range test ({alpha} = 0.05)

 


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Fig. 5 Levels of (A) putrescine and (B) spermidine in the crown nodules of Chippewa and Hardin soybeans, from 10 to 59 d after emergence (DAE). Bars with the same letter at each DAE are not significantly different using Duncan's multiple range test ({alpha} = 0.05)

 


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Fig. 6 Cytology of crown nodules of Chippewa and Hardin soybeans 38 and 45 DAE at 200x magnification. Transverse sections of nodules from (A) Hardin and (B) Chippewa 38 d after emergence (DAE). Longitudinal sections of nodules from (C) Hardin and (D) Chippewa 45 DAE. Sections stained with toluidine blue. Outer cortex (OC), nodule endodermis (NE), inner cortex (IC), infected cells (INC), uninfected cells (UIC) and vascular bundles (VB)

 





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