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Using Amplified Fragment Length Polymorphisms and Simple Sequence Length Polymorphisms to Identify Cultivars of Brown and White Milled Rice

^a Division of Plant Science, School of Biological Science, University Park, University of Nottingham, NG7 2RD, UK
^b IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Bristol, BS18 9AF, UK
^c USDA-ARS, 1509 Aggie Drive., Beaumont, TX 77713 USA

View larger version (26K): [in a new window]   Fig. 1 Phylogenetic analysis of 22 Basmati, U.S. long-grain, and non-U.S. long-grain cultivars (a) using all 29 SSLP markers, (b) using only eight of the SSLP markers, Waxy, M7, M16, M22, RM220, RM224, RM234, and RM55. Scale bars show genetic distance as calculated by the (µ)2 method of Goldstein et al. (1995)

View larger version (37K): [in a new window]   Fig. 2 Amplified fragment length polymorphism (AFLP) chromatogram showing runs of Jodon and Kaybonnet DNA using two independent DNA preparations run on different days using Mse + CAA and EcoRI + AAG primers. Chromatograms 1 and 3 = Jodon, Chromatograms 2 and 4 = Kaybonnet. Arrows indicate polymorphic peaks as follows: peaks that are polymorphic between the two cultivars consistently between duplicates (a); a peak that appears to be polymorphic between Jodon and Kaybonnet for Chromatograms 1 and 2, but the polymorphic peak is not present in the duplicate Jodon run on Chromatogram 3 (b); peaks of variable heights between the duplicate runs for Jodon (Chromatograms 1 and 3) (c)