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Molecular Mapping of Russian Wheat Aphid Resistance from Triticale Accession PI 386156

^a Texas A&M Univ., Southern Crop Improvement Facility, College Station, TX 77843-2123 USA
^b Texas A&M Univ. Research and Extension Center, P.O. Box 1658, Vernon, TX 76384 USA

View larger version (13K): [in a new window]   Fig. 1 RAPD pattern of the resistant parent PI 386156 (RP), susceptible parent NE88T222 (SP), and the most resistant and most susceptible F2 plants, using primer OP-M09. Lane numbers for resistant mad susceptible plants correspond to individual plant identification numbers given in Table 1

View larger version (53K): [in a new window]   Fig. 2 The DNA sequence analysis of the OP-M09220 fragment amplified from PI 386156 and NE88T222 is shown. Relative to PI 386156, there is a deletion of a `G' nucleotide in the fragment amplified from NE88T222. The arrow identifies the single nucleotide polymorphism

View larger version (49K): [in a new window]   Fig. 3 The STS primers derived from the sequence of OP-M09220 amplified a fragment from the resistant parent, PI 386156 (RP) and the 4R wheat-rye addition line. Lanes labeled 1 though 7 are wheat-rye addition lines for rye chromosomes 1R through 7R, respectively