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Molecular Mapping of the Rcs3 Gene for Resistance to Frogeye Leaf Spot in Soybean

^a Dep. of Crop and Soil Sciences, Univ. of Georgia, Athens, GA 30602-7272 USA
^b Dep. of Plant Pathology, Georgia Exp. Stn., Griffin, GA 30223 USA

View larger version (28K): [in a new window]   Fig. 1 Gel image (from an ABI Prism 377 DNA sequencer using GeneScan analysis) for Wright, Wright-Rcs3 NIL, Blackhawk, Davis, FLS resistant bulk, and FLS susceptible bulk DNA for SSR markers Satt244 and Satt431. The NED labeled Satt244 is shown in yellow and the 6-FAM labeled Satt431 is shown in blue. The in-lane size standards are shown in red and the estimated number of base pairs (bp) for each in-lane size standard is shown on the left hand side of the gel

View larger version (14K): [in a new window]   Fig. 2 The section of linkage group (LG) J of the Blackhawk x Davis population showing the position of molecular markers (SSR and RFLP) and the Rcs3 gene for resistance to frogeye leaf spot (FLS) in soybean. A marker name and position are shown on the left and the estimated map distances (cM) are shown on the right of the LG. A Satt or a Sct_ prefix indicates a SSR marker locus. An RFLP marker locus is identified with a probe designation (a capital letter code with a number code) followed by a dashed number code