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FISH and RFLP Marker-Assisted Introgression of Festuca mairei Chromosomes into Lolium perenne

^a Harvard Institute of Human Genetics, Harvard Medical School, 4 Blackfan Circle, Boston, MA 02146 USA
^b Dep. of Agronomy, Univ. of Missouri-Columbia, Columbia, MO 65211-0001 USA

View larger version (23K): [in a new window]   Fig. 1 Crossing scheme for introgression of Festuca mairei genomes (MMM'M') into Lolium perenne (LL). The 4x F1 hybrid, F. mairei (4x) x L. perenne (2x), was used as the female in backcrossing to L. perenne. Fourteen BC1 plants were interpollinated to produce BC1G1 progeny containing from chromosomes

View larger version (27K): [in a new window]   Fig. 2 Genomic FISH using labeled F. mairei DNA as a probe effectively detected substitution of a pair of Festuca mairei chromosomes in a Lolium-like hybrid plant

View larger version (12K): [in a new window]   Fig. 3 Distribution of the chromosome number among a total of 64 BC1G1 plants derived from Festuca mairei x Lolium perenne hybrids

View larger version (51K): [in a new window]   Fig. 4 Southern hybridization of the HindIII- or EcoRI-digested genomic DNA of Lolium perenne (Lp), Festuca mairei (Fm), and their hybrid derivatives with (A) TF109, identifying the presence of F. mairei genome in F1, F2, and BC1 plants; (B) TF515, a Festuca genome-specific probe, showing strong hybridization only in F1, BC1, and some BC1G1 plants (M = labeled DNA/HindIII fragments)