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Genetic Variability as Identified by AP-PCR and Reaction to Mid-Stem Infection of Sclerotinia sclerotiorum among Interspecific Sunflower (Helianthus annuus L.) Hybrid Progenies

^a Institute of Crop Science and Plant Breeding I, Faculty of Agriculture and Environment Preservation, Justus-Liebig-University, Ludwigstr. 23, D-35390 Giessen, Germany

View larger version (76K): [in a new window]   Fig. 1 Genotypic reaction of sunflower interspecific progenies to Sclerotinia after artificial mid-stem inoculation. Data shown as the extension of stem lesions (cm) determined the last scoring date. Interspecific genotypes that showed a significantly lower average level of Sclerotinia infection than Frankasol (# 14) are represented by black bars. For description of the genotypes see Table 1

View larger version (168K): [in a new window]   Fig. 2 AP-PCR fingerprint of interspecific progenies (H. annuus x H. tuberosus); PCR carried out with the primer AP17. Lanes 2, 3: parents of the interspecific cross (HA89cms, H. tuberosus1705). Lanes 4 through 17: interspecific progenies that were backcrossed to H. annuus twice and self-pollinated three times (BC2S3). Lanes 1 and 18: 100-bp ladder (Gibco BRL, Life Science Technology)

View larger version (26K): [in a new window]   Fig. 3 Dendrogram generated from AP-PCR data determined by the unweighted pair group method of arithmetic means (UPGMA). Data of seven wild Helianthus species, two sunflower inbred lines, three sunflower commercial hybrids, and 29 interspecific progenies (#2–6, 8–13 and 15–36; the wild species that were used for the initial crosses are noted in parenthesis) were analyzed. The scale is based on Nei and Li's coefficient of similarity