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Confirmation of QTL Associated with Common Bacterial Blight Resistance in Four Different Genetic Backgrounds in Common Bean

^a Dep. of Horticulture, Univ. of Wisconsin, Madison, WI 53706 USA
^b Washington Univ., School of Medicine, Dep. of Biochemistry and Molecular Biophysics, St. Louis, MO 63110 USA
^c Dep. of Horticulture, Univ. of Nebraska, Lincoln, NE 68583 USA
^d Centro de Investigaciones Agricola del Surocate (CIAS), San Juan de la Maguana, Dominican Republic

View larger version (71K): [in a new window]   Fig. 1 Frequency distributions of RI and BC2F3 line means for common bacterial blight (CBB) disease reactions in different plant organs. CBB resistance in first trifoliolate leaves and later developed trifoliolate leaves to Xcp strains was measured as the percentage of inoculated area with CBB symptoms which varied from 0.0 (resistant) to 100 (susceptible) or as the visual rating scales varied from 1 (resistant) to 5 (susceptible). CBB reaction in pods to Xcp strains was measured as the length (mm) of the water soaked region in inoculated pods and varied from 0.0 to 5.0. (Continued on next page.)

View larger version (20K): [in a new window]   Fig. 2 Two marker locus-QTL associations for resistance to Xanthomonas campestris pv. phaseoli (Xcp) previously identified in the BAC 6 x HT 7719 population across the RI populations of Venezuela 44 x BAC 6 (VB), Belneb RR-1 x A55 (BA), and BAC 6 x HT 7719 (BH) and in the BC2F3 population of PC 50 x BAC 6 (PB). The most likely locations of QTL based on single-factor ANOVA in each population are indicated by oval shapes. Markers common to linkage groups constructed for different populations are connected by lines

View larger version (79K): [in a new window]   Fig. 3 (a) RAPD amplification of six bean parents used for the development of four segregating common bean populations including the RI populations of Venezuela 44 x BAC 6 (VB), Belneb RR-1 x A55 (BA), and BAC 6 x HT 7719 (BH) and the BC2F3 population of PC 50 x BAC 6 (PB). Key to individuals: lane 1 a 100-bp molecular weight ladder, lane 2 `A55', lane 3 `Belneb RR-1', lane 4PC 50, lane 5 `Venezuela 44', lane 6 `BAC 6', and lane 7 `HT 7719'. The arrow indicates the RAPD band with molecular weight of approximately 1250 base pair (BC409.1250) which is only amplified in resistant parents, BAC 6 and Belneb RR-1. (b) Primer specific amplification of the BC409.1250 polymorphism based on PCR primers designed from the sequence of the RAPD fragment