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Published in Crop Sci 39:1444-1447 (1999)
© 1999 Crop Science Society of America
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Analysis of the Distribution of the Major Soybean Seed Allergens in a Core Collection of Glycine max Accessions

R.W. Yaklicha, R.M. Helmc, G. Cockrellc and E.M. Hermanb

a Soybean and Alfalfa Res. Lab., Beltsville, MDsc USA
b Climate Stress Lab., USDA-ARS, Beltsville Agric. Res. Center, scBeltsville, MDsc 20705 USA
c Dep. of Pediatrics, Univ. of Arkansas for Medical Sciences, Arkansas Children's Hospital Research Institute, Little Rock, AR 72202 USA



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Fig. 1 Immunoblot analysis of the seed extracts of soybean, Glycine max. Upper panel (A), amido-black stained blot of the total proteins. Middle panel (B), replicate blot that was treated with anti-P34 serum. Lower panel (C), replicate blot that was treated with human serum. Arrowheads (far left) are the molecular weight rainbow standards. Lanes 1–14 represent the lines `AK Harrow', `Arksoy', `Bansei', Black Eyebrow, Blackhawk, `Capital', `CNS', `Dunfield', `Flambeau', `Haberlandt', `Illini', `Improved Pelican', `Jackson', and `Jogun'

 


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Fig. 2 Immunoblot analysis of the seed extracts of soybean, Glycine max. Upper panel (A), amido-black stained blot of the total proteins. Middle panel (B), replicate blot that was treated with anti-P34 serum. Lower panel (C), replicate blot that was treated with human serum. Arrowheads (far left) are the molecular weight rainbow standards. Lanes 1–14 represent the lines D81-8359, CX804-108, NC-2-62, CX797-115, CX797-21, 76-48773, D81-8498, BARC-6, BARC-7, BARC-8, BARC-9, Williams, Miles, and Clark, respectively

 


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Fig. 3 Immunoblot analysis of the seed extracts of Glycine species. Upper panel (A), amido-black stained blot of the total proteins. Middle panel (B), replicate blot that was treated with anti-P34 serum. Lower panel (C), replicate blot that was treated with human serum. Arrowheads (far left) are the molecular weight rainbow standards. Lanes 1–14 represent G. arenaria (PI 505204), G. argyrea (PI 505151, PI 509451), G. canescens (PI 440932, PI 440942, PI 446934), G. clandestina (PI 440948, PI 440954, PI 440960), G. curvata (PI 505164, PI 505166, PI 505167), and G. cyrtoloba (PI 440962, PI 440963), respectively

 





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