Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 9:810-812 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Sweetclover Weevil Feeding Stimulants: Isolation and Identification of Glucose, Fructose, and Sucrose1

W. R. Akeson, H. J. Gorz and F. A. Haskins2

The water-soluble fraction of Melilotus leaves previously referred to as Stimulant A was further fractionated by preparative paper chromatography into three factors, each having a stimulatory effect on sweetclover weevil feeding. The three factors, designated Stimulant A1, A2, and A3, were identified as sucrose, glucose, and fructose, respectively, by co-chromatography with known sugars on cellulose-coated thin-layer plates. Silver nitrate, anisidine phthalate, and anthrone were employed as detection agents. The isolated compounds and corresponding reagent grade sugars were identical in chromatographic and chemical behavior, as well as in feeding stimulant activity. Sucrose stimulated the greatest amount of feeding when the three sugars were compared at equal concentration in the same bioassay. Disks treated with glucose and fructose were fed upon equally at the three concentrations tested. Although these sugars probably play a significant role in the mechanism of resistance and susceptibility of Melilotus plants to weevil feeding, it is not likely that they are wholly responsible for the host specificity displayed by tire sweetclover weevil.

Key Words: Sitona cylindricollis • Melilotus officinalis • Melilotus infesta

1 Contribution from the Nebraska Agricultural Experiment Station, Lincoln, Nebraska, and the Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture. Supported in part by Crops Research Division and Entomology Research Division, Agric. Res. Serv., USDA Grant No. 12-14-100-8027 (33). Published with the approval of the Director as Paper No. 2560, Journal Series, Nebraska Agr. Exp. Sta.

2 Assistant Professor of Agronomy, University of Nebraska; Research Geneticist, Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture; and Bert Rodgers Professor of Agronomy, University of Nebraska, respectively, Lincoln, Nebraska, 68503. The technical assistance of Patricia Underwood and Henry J. Stevens is gratefully acknowledged.

Received for publication May 26, 1969.




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Environmental Quality		 The Plant Genome
Copyright © 1969 by the Crop Science Society of America.