HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Jones, M. Articles by Elliot, F. C. Search for Related Content PubMed Articles by Jones, M. Articles by Elliot, F. C. Agricola Articles by Jones, M. Articles by Elliot, F. C.

Two Rapid Assays for Saponin in Individual Alfalfa Plants¹

Two assays were developed for screening individual alfalfa (Medicago saliva) plants for crude saponin. Twenty alfalfa leaf samples, selected on the basis of erythrocyte assay results, were evaluated by the fish assay and secondly by a chemical determination for saponins. The correlation coefficients of these assay results were erythrocyte vs. chemical (r = .90), fish vs. erythrocyte (r = –.91), and fish vs. chemical (r = –.81). Results indicate that the two assays may provide rapid and accurate estimates of crude saponin in alfalfa plants.

Key Words: Forage quality • Hemolysis • Alfalfa saponin • Fish assay • Erythrocyte assay

² Formerly, NDEA Fellow at Michigan State University (now Senior Biologist for Monsanto Company, St. Louis, Mo.) and Professor, Department of Crop Science, Michigan State University, East Lansing Mich. 48823.

Received for publication January 7, 1969.