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Four additional isozyme differences among the I2 inbred parents of two reciprocal recurrent selection populations (A and B) of maize arc described. Some common problems in the resolution of mobility differences are exemplified by the properties of these systems. Evidence is cited which shows that the mobility differences between single bands in these systems arise from codominant allelic variation at single loci. Data are reported concerning the linkage relationships of these four loci with each other, and with five previously described isozyme loci. Individual seedlings can be scored for all nine loci simultaneously, and therefore can be assigned to one of many thousand potential genotypic classes in monitoring the genetic effects of this selection regime.
Key Words: Isozyme resolution prolems Acetate esterases Butyrate esterase Phosphatase Linked isozyme markers
2 Research Assistant, Department of Agronomy and Range Science, and Professor of Genetics.
Received for publication March 20, 1969.
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