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Individual alfalfa clones derived from populations of Medicago sativa L., M. falcata L., M. glutinosa M. B., and M. coerulea Less. were analyzed for nutritive value by using (a) in vitro fermentation techniques and (b) fiber analyses.
Considerable variation in estimated nutritive value occurred both within and between populations. The range of values of percent dry matter disappearance (DMD), using a 6-hr in vitro fermentation technique, within populations was from 45.0 to 27.1% (M. sativa, DuPuits) to 33.3 to 27.7% (M. glutinosa). The corresponding range between populatiou means was 35.4 (M. sativa, DuPuits) to 26.3% (M. falcata, Wisconsin 460). Clones of high estimated uutritive value were characterized by being relatively low in fibrous or cell wall constituents. Similarly, the production of acetic, propionic, and butyric acids was consistently greater in fermentations when clones of l~igh estimated nutritive value were used as substrates compared to clones of low estimated nutritive value.
Key Words: Dry matter disappearance Estimated nutritive value Clones Cell wall constituents Volatile fatty acids.
2 Former graduate assistant in Crop Science (now Assistant Professor of Agronomy, Department of Plant Science, University of Connecticut, Storrs 06268) and Professors of Crop Science.
Received for publication March 14, 1969.
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