Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 9:489-491 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Genetic Differences in Soybean Trypsin Inhibitors Separated by Disc Electrophoresis1

Laxman Singh, C. M. Wilson and H. H. Hadley2

The acid-soluble whey proteins from seeds of several varieties of soybeans were analyzed by disc electrophoresis. Almost all varieties had a very intense protein band just behind the front which corresponded with the major band of commercial soybean trypsin inhibitor. The lines L63-9485 and T-245 lacked this band, but had a slightly slower moving band. The F1 seed of reciprocal crosses between L63-9485 and Clark possessed both intense bands, while the F2 seed segregated to show one or the other or both bands. The gels were sliced and extracted with buffer. Trypsin inhibitor was recovered from the area of both intensely stained bands.

Key Words: Glycine max (L.) Merr.


1 Contribution from the Department of Agronomy, University of Illinois, and the Crops Research Division, ARS, USDA, Urbana, Illinois 61801.

2 Rockefeller Foundation Fellow in Plant Genetics; Research Chemist, Crops Research Division, ARS, USDA; and Professor of Plant Genetics, University of Illinois, Urbana, Illinois, repectively.

Received for publication February 5, 1969.


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