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Published in Crop Sci 9:389-390 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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In Vitro Germination of Kentucky Bluegrass Pollen (Poa Pratensis L. ‘Newport’)1

M. Anwar Maun, I. D. Teare and C. L. Canode2

The germination of Kentucky bluegrass pollen, Poa pratensis L., ‘Newport’, was studied in artificial cultures containing variable concentrations of sucrose, calcium nitrate and boric acid. The germinations were made in an incubator in the dark at 21 ± 1 C. Good germination and growth were obtained with 13% sucrose, 0.75% agar agar, and 10 ppm of calcium nitrate and 5 ppm of boric acid.

Key Words: Sucrose • Calcium • Boron

1 Cooperative investigations of the Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, and Washington Agricultural Experiment Stations. Scientific Paper No. 3175. College of Agriculture, Washington State University, Pullman 99163. Supported in part by Hatch and State Project 1797.

2 Senior lecturer, West Pakistan Agricultural University, Lyallpur, Formerly Graduate Student, Washington State University; Assistant Professor of Agronomy, Washington State University; Research Agronomist, Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture.

Received for publication November 21, 1968.




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