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Contamination in Denta Sweetclover During Successive Generations of Seed Increase¹

Contamination levels observed in the breeder, foundation, and certified seed generations of ‘Denta’ sweetclover (Melilotus alba Desr.) were 0.71, 1.69, and 3.62%, respectively, as measured by the frequency of plants high in content of o-hydroxycinnamic acid. Based on a sample of plants tested simultaneously for o-hydroxycinnamic acid content and ß-glucosidase activity, a small but consistent decrease in the frequency of cu plants occurred with succeeding seed generations, while shifts in the frequency of b plants were not consistent in magnitude or direction. Contamination through cross-pollination with CuCuBB plants may be the major factor responsible for the observed decreases in the frequency of cu plants.

Key Words: Seed contamination • Genetic shift • o-hydroxycinnamic acid

² Research Geneticist, Crops Research Division, Agricultural Research Service, U. S. Department of Agriculture, and Bert Rodgers Professor of Agronomy, University of Nebraska.

Received for publication December 20, 1968.