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Published in Crop Sci 9:346-348 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Plant Differentiation from Callus Tissue of Saccharum Species1

Don J Heinz and Grace W. P. Mee2

Callus tissue was induced on parenchyma tissue of shoot apices, leaves, and inflorescences of Saccharum sp. using a basal mineral medium supplemented with coconut water (10% by volume) and 2,4-D. Transfer of callus tissue to a non-2,4-D basal medium induced the differentiation of plants. In all the plants studied, morphological variants from the parental clone were observed.

Key Words: Chromosome variability • Plant variability • Tissue culture • Isozyme patterns • Induced differentiation • Selection technique • Aneuploidy

1 Published with the approval of the Director as Paper No. 231 in the Journal Series of the Experiment Station, Hawaiian Sugar Planters' Association

2 Respectively, Head of Genetics Department, and Junior Scientist, Genetics Department, Experiment Station, Hawaiian Sugar Planters' Association, Honolulu, Hawaii 96822.

Received for publication November 12, 1968.




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Copyright © 1969 by the Crop Science Society of America.