Crop Science Journal of Natural Resources and Life Sciences Education
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Published in Crop Sci 9:173-176 (1969)
© 1969 Crop Science Society of America
677 S. Segoe Rd., Madison, WI 53711 USA
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Semi-Vitro Methods in the Study of Compatibility in Birdsfoot Trefoil (Lotus corniculatus L.)1

Ludwik Spiss and Dominick J. Paolillo, Jr.2

In semi-vitro cultures, there were no differences in emergence of pollen tubes from styles of birdsfoot trefoil (Lotus cornlculatus L.) after cross- and self-pollinations. These findings support the hypothesis that compatibility of a mating in birdsfoot trefoil is not determined by differential growth rates of cross- and self-pollen tubes through the style. The differential in performance of pollen tubes is presumed to be manifested in the ovary. However, preliminary tests for chemotropic responses of pollen tubes to ovarian tissues have failed to reveal how this differential performance is regulated.

Key Words: Self-sterility • Incompatibility


1 Contribution from the Departments of Agronomy and Botany, University of Illinois, Urbana 61801. Based on a part of the dissertation submitted by the senior author in partial fulfillment of the requirements for the Ph.D. degree in Agronomy, University of Illinois, Urbana

2 Formerly Research Assistant, Department of Agronomy, University of Illinois (now Plant Breeder, Department of Plant Breeding, College of Agriculture, Cracow, Lobzowska 24, Poland), and Associate Professor of Botany, University of Illinois, Urbana 61801.

Received for publication July 11, 1968.





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