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Chromosomal Location of Rf₂, a Restorer Gene for Cytoplasmic Pollen Sterile Maize¹

Rf₂, one of two major dominant genes needed to restore pollen fertility to the T-type of cytoplasmic pollen sterile maize (Zea mays L.), has been shown to be located on chromosome 9, approximately 5 crossover units from waxy endosperm (wx) and adjacent to the centromere. Results from tests involving chromosomal translocations and tests involving marker genes were in complete agreement.

Key Words: Translocations • Linkage testers • Gene placement

² Department of Horticulture, University of Maryland, College Park 20740, and Department of Plant Breeding, Pioneer Hi-Bred Corn Co., Johnston, Iowa.

Received for publication June 22, 1968.