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Evaluation of the breeding behavior of two Arena 6x amphiploids constituted the initial phase of a study of their usefulness in facilitating transfer of genes from diploids and tetraploids to hexaploids. Most amphiploid seedling root tip cells in 33 A6 to A9 lines contained from 38 to 44 chromosomes. The most frequent number was 42. Chromosome numbers varied among cells in the same root tip and among tips from sister seedlings. Lines which were uniform and stable for high self-fertility were not found, even after 13 generations. While segregation for reaction to crown rust continued in some A0 lines, others tested in A12 were homogeneous for resistance. A seed set of 20.8% (383 kernels from 1,838 florets) was obtained in amphiploid
x A. sativa L.
pollinations made in the greenhouse. Even though these amphiploids are not agronomically desirable, their relatively high compatibility with A. sativa gives hope for their usefulness.
Key Words: Oats Plant breeding Gene transfer Interspecific hybridization Chromosome-number mosaics Sterility Crown rust resistance Compatibility Instability
2 Assistant Professor and Professor of Agronomy, University of Wisconsin, lVladison 53706.
Received for publication June 12, 1968.
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